Screening of self-assembled monolayer for aflatoxin B1 detection using immune-capacitive sensor

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Screening of self-assembled monolayer for aflatoxin B1 detection using immune-capacitive sensor

Alvaro V. Gutierrez Ra,b, Martin Hedströma,c, Bo Mattiassona,c,*
a Department of Biotechnology, Lund University, Lund, Sweden
b IIFB, FCFB, Universidad Mayor de San Andres, La Paz, Bolivia
c CapSenze HB, Medicon Village, Lund, Sweden

ARTICLEINFO
Article history: Received 16 July 2015
Received in revised form 23 September 2015
Accepted 4 October 2015
Available online 27 October 2015
Keywords: Capacitive Sensor Aflatoxin Self-assembled monolayer Capacitance

ABSTRACT
A capacitive biosensor was used for detection of aflatoxin B1. Two different methods for cleaning gold electrodes were evaluated using cyclic voltammetry in the presence of ferricyanide as redox couple. The methods involve use of a sequence of cleaning steps avoiding the use of Piranha solution and plasma cleaner. Anti-aflatoxin B1 was immobilized on self-assembled monolayers (SAM). The immune-capacitive biosensor is able to detect aflatoxin B1 concentrations in a linear range of 3.2 _ 10_12M to 3.2 _ 10_9M when thiourea was used to form the SAM; 3.2 _ 10_9M to 3.2 _ 10_7M when thioctic acid was used. When the gold surface was isolated with tyramine-electropolymerization linear ranges of 3.2 _ 10_13M to 3.2 _ 10_7M and 3.2 _ 10_9M to 3.2 _ 10_7M where obtained, respectively. The results obtained show the difference in linear range, limit of detection, and limit of quantification when different self-assembled monolayers are used for aflatoxin B1 detection. ã _2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license

http://dx.doi.org/10.1016/j.btre.2015.10.001