CapSenze Flow System

  • Overview

    The Capsenze Flow System is designed to sample a bioreactor and automatically digest and separate individual antibody subdomains for direct analysis by mass spectrometry and/or other technologies. The system is built on CapsSenze´s unique liquid handling system and adapted for use with Genovis enzymes.

    The system automatically samples from the bioreactor and process the mAb to provide subunits, utilizing affinity matrices and unique enzymes integrated into a disposable column cartridge. As a result, it is possible to characterize the whole mAb via fragments analyse done-by-one, thus enabling a very high level of quality assurance directly during production.

     

  • Technical Details & Applications


    Integrated CapSenze
    Flow System:

    • Dimensions 85 × 65 × 30 cm
    • Operated using single syringe pumps and and smart multiport valves from Tecan
    • Controlled by CapSenze software
    • The serial columns designed in a user-friendly cassette format

     

    Capsenze Flow System details

    • Pump and valve system – OEM/Tecan
    • Sample probe for online bioreactor sampling (CapSenze)
    • Microfluidic enzyme cassette system – (CapSenze/Genovis)
    • Flexible bag system for liquid handling (disposable)
    • Integrated flow-through UV-detector (OEM/Visacon)
    • Inline desalting system for sample cleanup prior to MS
    • Web-based software (CapSenze)

  • Learn more

    Kumar, M.A., Mazlomi, M., Hedström, M., Mattiasson B. Versatile Automated Continuous Flow System (VersAFlo) for Bioanalysis and Bioprocess Control. Sensors and Actuators B: Chemical, 2012 161, 855-861.

    Mazlomi, M., Hedström, M., Mattiasson B. Integrated set-up of sampling, sample treatment and assay of an intracellular protein during fermentation. Journal of Biotechnology, 2010, 150, 366–371.

    Mazlomi, M., Hedström, M., Mattiasson B. Optimizing induction during transgenic protein production using VersAFLo, a fully automated on-line monitoring and control system. Submitted.

    Zór, K., Jantra J., Olsson F., Erlandsson D., Fredriksson S., Wiechmann A., Suckau D., Tremtin G., Wood J., Hedström M., Jabs W. Automated flow-based analytical platform for controlled monoclonal antibody fragmentation and glycoprofiling using ultrahigh-resolution QTOF mass spectrometry. Manuscript in preparation.