The Capsenze Flow System is designed to sample a bioreactor and automatically digest and separate individual antibody subdomains for direct analysis by mass spectrometry and/or other technologies. The system is built on CapsSenze´s unique liquid handling system and adapted for use with Genovis enzymes.
The system automatically samples from the bioreactor and process the mAb to provide subunits, utilizing affinity matrices and unique enzymes integrated into a disposable column cartridge. As a result, it is possible to characterize the whole mAb via fragments analyse done-by-one, thus enabling a very high level of quality assurance directly during production.
Integrated CapSenze Flow System:
Capsenze Flow System details
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Mazlomi, M., Hedström, M., Mattiasson B. Optimizing induction during transgenic protein production using VersAFLo, a fully automated on-line monitoring and control system. Submitted.
Zór, K., Jantra J., Olsson F., Erlandsson D., Fredriksson S., Wiechmann A., Suckau D., Tremtin G., Wood J., Hedström M., Jabs W. Automated flow-based analytical platform for controlled monoclonal antibody fragmentation and glycoprofiling using ultrahigh-resolution QTOF mass spectrometry. Manuscript in preparation.