Microcontact imprinting based biosensor for ultrasensitive detection of prostatespecific antigen (PSA)

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Microcontact imprinting based surface plasmon resonance (SPR) biosensor for real-time and ultrasensitive detection of prostate specific antigen (PSA) from clinical samples

Gizem Ertürk a, Haluk Özen b, M. As¸ kın Tümer a, Bo Mattiasson c, Adil Denizli d,∗
a Hacettepe University, Department of Biology, Ankara, Turkey
b Hacettepe University, Department of Urology, Ankara, Turkey
c Lund University, Department of Biotechnology, Lund, Sweden
d Hacettepe University, Department of Chemistry, Ankara, Turkey

ARTICLE INFO
Article history:
Received 10 August 2015
Received in revised form 23 October 2015
Accepted 26 October 2015
Available online 7 November 2015
Keywords:Prostate specific antigen, Surface plasmon resonance, Microcontact imprinting, Enzyme-linked immunosorbent assay

ABSTRACT
Prostate-specific antigen (PSA) is an important biomarker for diagnosis and prognosis of prostate cancer. Herein, microcontact PSA-imprinted surface plasmon resonance (SPR) sensor chip was developed for sensitive, real-time detection of PSA. The imprinted chip was prepared in the presence of methacrylic acid(MAA) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker via UV polymerization using microcontact imprinting technique. PSA imprinted SPR sensor chip was characterized by atomic force microscope (AFM), scanning electron microscope (SEM), ellipsometry, dispersive Ramanand Fourier transform infrared spectroscopy (FT-IR). Under optimal conditions, PSA detection was performed with standard PSA solutions in the concentration range of 0.1–50 ng mL−1with a detection limit(LOD) of approximately 91 pg mL−1(18 × _10−14M). Selectivity studies were performed against human serum albumin (HSA) and lysozyme (Lyz) as the competitive agents. The developed system was evaluated for analysis of 10 clinical serum samples and showed approximately 98% agreement between the results obtained by commercial enzyme-linked immunosorbent assay (ELISA) method without significant differences at the 0.05 significance level (p = 0.751, p > 0.05).

http://dx.doi.org/10.1016/j.snb.2015.10.093