Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

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Affinity sensor based on immobilized molecular imprinted synthetic recognition elements

Pieterjan Lenain a,n, Sarah De Saeger a, Bo Mattiasson b, Martin Hedström b
a Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent,Belgium
b Capsenze HB, Medicon Village, SE-22381 Lund, Sweden

ARTICLEINFO
Article history: Received 19 November 2014
Received in revised form 30 January 2015
Accepted 10 February 2015
Available online 11 February 2015

Keywords: Molecularly imprinted polymer Capacitive affinity sensor Monitoring sensors Analytical methods Metergoline

ABSTRACT
An affinity sensor based on capacitive transduction was developed to detect a model compound, metergoline, in a continuous flow system. This system simulates the monitoring of low-molecular weight organic compounds in natural flowing waters, i.e. rivers and streams. During operation in such scenarios, control of the experimental parameters is not possible, which poses a true analytical challenge. A two- step approach was used to produce a sensor for metergoline. Submicron spherical molecularly imprinted polymers, used as recognition elements, were obtained through emulsion polymerization and subsequently coupled to the sensor surface by electropolymerization. This way, a robust and reusable sensor was obtained that regenerated spontaneously under the natural conditions in a river. Small organic compounds could be analysed in water without manipulating the binding or regeneration conditions, thereby offering a viable tool for on-site application

http://dx.doi.org/10.1016/j.bios.2015.02.016