Bioimprinting as a tool for the detection of aflatoxin B1 using a capacitive biosensor

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Bioimprinting as a tool for the detection of aflatoxin B1 using a capacitive biosensor

Alvaro V. Gutierrez R.a,b, Martin Hedströma,c, Bo Mattiassona,c,*

a Division of Biotechnology, Lund University, Lund, Sweden

b IIFB, FCFB, Universidad Mayor de San Andres, La Paz, Bolivia

c  CapSenze Biosystems AB, Scheelevägen 22, Lund, Sweden

 

ARTICLEINFO
Article history: Received 5 April 2016
Received in revised form 13 May 2016
Accepted 13 May 2016
Available online 25 May 2016
Keywords: Imprinted protein Bioimprinting Capacitance Aflatoxin

ABSTRACT
A strategy for the detection of aflatoxin B1 using a capacitive biosensor has been studied. The use of proteins for the generation of sites with high specificity against aflatoxin B1 are produced via bioimprinting. This technique has become a tool for the detection of aflatoxin B1 using a capacitive biosensor. The results demonstrate the ability to generate specific interactions with aflatoxin B1 with a linear relation between signals registered and log concentration of the target aflatoxin in the concentration range of 3.2  _ 10 _6 to 3.2  _ 10 _9M when using ovalbumin as a framework for the bioimprinting. ã _2016 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

 

http://dx.doi.org/10.1016/j.btre.2016.05.006